Regulatory

Part:BBa_K3505015

Designed by: Venetios Michelioudakis, Magdalini Koroxenidou, Foteini Papadaki   Group: iGEM20_Thessaly   (2020-10-20)


prpBCDE:RBS GB compatible with A1-B2

Level 0 prpBCDE with RBS BBa_B0034

Usage and Biology

The prpBCDE is a SCFAs inducible promoter and more specifically from Acetate and Propionate[1]. From the prpBCDE operon at Salmonella typhimurium LT2.

Fig.1:prpBCDE:RBS

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007and has overhangs compatible for GoldenBraid cloning. The Promoter has position A1-B3.

Fig.2:The overhangs of this part in the GoldenBraid Grammar

Verification of cloning

Fig.3:U=uncut C= cut ,Digestion with EcoRI. Expected band at 2297.C3 positive

Experimental Use and Experience

This part is used in BBa_K3505031

Source

Synthesized by IDT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  • [1]Tsang, A., Horswill, A. and Escalante-Semerena, J., 1998. Studies of Regulation of Expression of the Propionate (prpBCDE) Operon Provide Insights into How Salmonella typhimurium LT2 Integrates Its 1,2-Propanediol and Propionate Catabolic Pathways. Journal of Bacteriology, 180(24), pp.6511-6518.
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Parameters
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